298 research outputs found
Localized Joule heating produced by ion current focusing through micron-size holes
We provide an experimental demonstration that the focusing of ionic currents
in a micron size hole connecting two chambers can produce local temperature
increases of up to C with gradients as large as K. We find a good agreement between the measured temperature profiles and
a finite elements-based numerical calculation. We show how the thermal
gradients can be used to measure the full melting profile of DNA duplexes
within a region of 40 m. The possibility to produce even larger gradients
using sub-micron pores is discussed.Comment: 3 pages, accepted to Appl. Phys. Lett
Unzipping Dynamics of Long DNAs
The two strands of the DNA double helix can be `unzipped' by application of
15 pN force. We analyze the dynamics of unzipping and rezipping, for the case
where the molecule ends are separated and re-approached at constant velocity.
For unzipping of 50 kilobase DNAs at less than about 1000 bases per second,
thermal equilibrium-based theory applies. However, for higher unzipping
velocities, rotational viscous drag creates a buildup of elastic torque to
levels above kBT in the dsDNA region, causing the unzipping force to be well
above or well below the equilibrium unzipping force during respectively
unzipping and rezipping, in accord with recent experimental results of Thomen
et al. [Phys. Rev. Lett. 88, 248102 (2002)]. Our analysis includes the effect
of sequence on unzipping and rezipping, and the transient delay in buildup of
the unzipping force due to the approach to the steady state.Comment: 15 pages Revtex file including 9 figure
Inferring DNA sequences from mechanical unzipping: an ideal-case study
We introduce and test a method to predict the sequence of DNA molecules from
in silico unzipping experiments. The method is based on Bayesian inference and
on the Viterbi decoding algorithm. The probability of misprediction decreases
exponentially with the number of unzippings, with a decay rate depending on the
applied force and the sequence content.Comment: Source as TeX file with ps figure
Probing the mechanical unzipping of DNA
A study of the micromechanical unzipping of DNA in the framework of the
Peyrard-Bishop-Dauxois model is presented. We introduce a Monte Carlo technique
that allows accurate determination of the dependence of the unzipping forces on
unzipping speed and temperature. Our findings agree quantitatively with
experimental results for homogeneous DNA, and for -phage DNA we
reproduce the recently obtained experimental force-temperature phase diagram.
Finally, we argue that there may be fundamental differences between {\em in
vivo} and {\em in vitro} DNA unzipping
Unzipping DNA with Optical Tweezers: High Sequence Sensitivity and Force Flips
AbstractForce measurements are performed on single DNA molecules with an optical trapping interferometer that combines subpiconewton force resolution and millisecond time resolution. A molecular construction is prepared for mechanically unzipping several thousand-basepair DNA sequences in an in vitro configuration. The force signals corresponding to opening and closing the double helix at low velocity are studied experimentally and are compared to calculations assuming thermal equilibrium. We address the effect of the stiffness on the basepair sensitivity and consider fluctuations in the force signal. With respect to earlier work performed with soft microneedles, we obtain a very significant increase in basepair sensitivity: presently, sequence features appearing at a scale of 10 basepairs are observed. When measured with the optical trap the unzipping force exhibits characteristic flips between different values at specific positions that are determined by the base sequence. This behavior is attributed to bistabilities in the position of the opening fork; the force flips directly reflect transitions between different states involved in the time-averaging of the molecular system
Electron-Phonon Interacation in Quantum Dots: A Solvable Model
The relaxation of electrons in quantum dots via phonon emission is hindered
by the discrete nature of the dot levels (phonon bottleneck). In order to
clarify the issue theoretically we consider a system of discrete fermionic
states (dot levels) coupled to an unlimited number of bosonic modes with the
same energy (dispersionless phonons). In analogy to the Gram-Schmidt
orthogonalization procedure, we perform a unitary transformation into new
bosonic modes. Since only of them couple to the fermions, a
numerically exact treatment is possible. The formalism is applied to a GaAs
quantum dot with only two electronic levels. If close to resonance with the
phonon energy, the electronic transition shows a splitting due to quantum
mechanical level repulsion. This is driven mainly by one bosonic mode, whereas
the other two provide further polaronic renormalizations. The numerically exact
results for the electron spectral function compare favourably with an analytic
solution based on degenerate perturbation theory in the basis of shifted
oscillator states. In contrast, the widely used selfconsistent first-order Born
approximation proves insufficient in describing the rich spectral features.Comment: 8 pages, 4 figure
Carrier relaxation in GaAs v-groove quantum wires and the effects of localization
Carrier relaxation processes have been investigated in GaAs/AlGaAs v-groove
quantum wires (QWRs) with a large subband separation (46 meV). Signatures of
inhibited carrier relaxation mechanisms are seen in temperature-dependent
photoluminescence (PL) and photoluminescence-excitation (PLE) measurements; we
observe strong emission from the first excited state of the QWR below ~50 K.
This is attributed to reduced inter-subband relaxation via phonon scattering
between localized states. Theoretical calculations and experimental results
indicate that the pinch-off regions, which provide additional two-dimensional
confinement for the QWR structure, have a blocking effect on relaxation
mechanisms for certain structures within the v-groove. Time-resolved PL
measurements show that efficient carrier relaxation from excited QWR states
into the ground state, occurs only at temperatures > 30 K. Values for the low
temperature radiative lifetimes of the ground- and first excited-state excitons
have been obtained (340 ps and 160 ps respectively), and their corresponding
localization lengths along the wire estimated.Comment: 9 pages, 8 figures, submitted to Phys. Rev. B Attempted to correct
corrupt figure
Multiband theory of multi-exciton complexes in self-assembled quantum dots
We report on a multiband microscopic theory of many-exciton complexes in
self-assembled quantum dots. The single particle states are obtained by three
methods: single-band effective-mass approximation, the multiband
method, and the tight-binding method. The electronic structure calculations are
coupled with strain calculations via Bir-Pikus Hamiltonian. The many-body wave
functions of electrons and valence holes are expanded in the basis of
Slater determinants. The Coulomb matrix elements are evaluated using statically
screened interaction for the three different sets of single particle states and
the correlated -exciton states are obtained by the configuration interaction
method. The theory is applied to the excitonic recombination spectrum in
InAs/GaAs self-assembled quantum dots. The results of the single-band
effective-mass approximation are successfully compared with those obtained by
using the of and tight-binding methods.Comment: 10 pages, 8 figure
Transient current spectroscopy of a quantum dot in the Coulomb blockade regime
Transient current spectroscopy is proposed and demonstrated in order to
investigate the energy relaxation inside a quantum dot in the Coulomb blockade
regime. We employ a fast pulse signal to excite an AlGaAs/GaAs quantum dot to
an excited state, and analyze the non-equilibrium transient current as a
function of the pulse length. The amplitude and time-constant of the transient
current are sensitive to the ground and excited spin states. We find that the
spin relaxation time is longer than, at least, a few microsecond.Comment: 5 pages, 3 figure
- …